›› 2012, Vol. 24 ›› Issue (1): 20-24.doi: 10.3969/j.issn.1004-616x.2012.01.005

• 论著 • 上一篇    下一篇

连翘提取物体外抗肿瘤活性的初步研究

颜 晰，赵连梅，孙佳玮，单保恩   

  1. 1. 河北医科大学第四医院科研中心，河北 石家庄 050011；2. 河北医科大学第一医院呼吸科，河北 石家庄 050011
  • 收稿日期:2011-05-09 修回日期:2011-10-02 出版日期:2012-01-30 发布日期:2012-01-30
  • 通讯作者: 单保恩

In vitro anti-tumor activity of Forsythia suspensa extract

YAN Xi，ZHAO Lian-mei，SUN Jia-wei，SHAN Bao-en   

  1. 1. Research Center, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011; 2. The Department Of Pulmonology, the First Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China
  • Received:2011-05-09 Revised:2011-10-02 Online:2012-01-30 Published:2012-01-30
  • Contact: SHAN Bao-en

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摘要： 目的： 观察连翘根、叶和果3个部位各自的醇提物和水提物对食管癌细胞体外增殖和凋亡的影响，探讨连翘根醇提物(ethanol extract of Forsythia suspensa root，FSEER)的抗肿瘤机制。方法：分别制备连翘根、叶和果3个部位的醇提物和水提物，用MTT法检测6种连翘提取物对食管癌细胞TE-1、TE-13、Yes-2、Eca-109的增殖抑制作用；然后用不同浓度FSEER处理食管癌细胞24 h后用倒置相差显微镜和瑞氏-吉姆萨染色观察细胞的形态学变化，再分别用不同浓度FSEER处理TE-13细胞不同时间后，以Annexin V/PI双染法通过流式细胞仪检测TE-13细胞凋亡率的变化，Western blot检测PARP蛋白表达变化。结果：6种连翘提取物对食管癌TE-13细胞均有增殖抑制作用(P＜0.05)，其中FSEER效果最明显。经FSEER处理后，TE-13细胞显示典型的凋亡形态变化，与对照组相比凋亡率明显增高(P＜0.05)，PARP蛋白出现了裂解片段的表达，且呈现一定的剂量和时间依赖性。结论：FSEER体外对人食管癌细胞的增殖有明显的抑制作用，其抗肿瘤机制可能与诱导细胞凋亡有关。

关键词: 连翘, 抗肿瘤, 增殖抑制, 细胞凋亡

Abstract: OBJECTIVE: To observe the effects of the ethanol and water extracts of Forsythia suspensa in which root，stem and leaf were tested， on the proliferation and apoptosis of human esophageal carcinoma cells in vitro， and to investigate its probable mechanisms. METHODS： The ethanol and water extracts of the root ，leaf and fruit of Forsythia suspensa were prepared. The growth-inhibitory effect of extracts of Forsythia suspensa on esophageal cancer cells were examined by MTT assay. Morphological changes of cancer cells were studied by light microscope and Wright-Giemsa staining. The apoptotic rate was analyzed with Annexin/PI double staining. Western blotting was employed to assess the changes of PARP expression. RESULTS ： All extracts of Forsythia suspensa could inhibit the proliferation of esophageal cancer cells，and the inhibitory effect of FSEER was the most obvious among them. After treatment with FSEER，TE-13 cells showed typical apoptosis morphological changes and the apoptotic rate was increased as compared to control group (P<0.05). Meanwhile， the cleavage of PARP was visible， and all of these changs were in a time-and dose-dependent manner. CONCLUSION： FSEER could significantly inhibit human esophageal carcinoma cell growth in vitro by inducing apoptosis.

Key words: Forsythia suspensa, anti-tumor, anti-proliferation, apoptosis

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